The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied utilizing Nycodenz/sucrose gradients. It was proven in pulse chase experiments that the ligand endocytosed initially
Day: March 14, 2021
Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.
Commonly, subcellular organelles equivalent to nuclei, mitochondria, lysosomes, and Golgi membranes are remoted first by differential centrifugation in low-speed or high-speed centrifuges after which purified