One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles.

One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles.

In the current examine, we describe a new process using freezing-thawing to density gradient resolution of Nycodenz for one-step separation of organelles from the rat liver and subsequent proteome evaluation of subcellular fractions. To put together two-dimensional electrophoresis (2-D PAGE) profiles of tissue organelles, we carried out one-step subcellular fractionation of rat liver homogenate using …

One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles. Read More »