Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.

Commonly, subcellular organelles equivalent to nuclei, mitochondria, lysosomes, and Golgi membranes are remoted first by differential centrifugation in low-speed or high-speed centrifuges after which purified by gradient centrifugation in ultracentrifuges. We have ready these organelles using a brand new high-speed centrifuge (28,000 rpm max) which permits the era of larger radial centrifugal forces (rcfs) than …

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge. Read More »