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Separation of endocytic vesicles in Nycodenz gradients.

Separation of endocytic vesicles in Nycodenz gradients.

The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied utilizing Nycodenz/sucrose gradients. It was proven in pulse chase experiments that the ligand endocytosed initially (after half of to 1 min) was in small, slow-sedimenting vesicles of comparable sizes. The vesicles containing the ligand elevated in measurement, and after about 2.5 min 20-30% of the …

Separation of endocytic vesicles in Nycodenz gradients. Read More »

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.

Commonly, subcellular organelles equivalent to nuclei, mitochondria, lysosomes, and Golgi membranes are remoted first by differential centrifugation in low-speed or high-speed centrifuges after which purified by gradient centrifugation in ultracentrifuges. We have ready these organelles using a brand new high-speed centrifuge (28,000 rpm max) which permits the era of larger radial centrifugal forces (rcfs) than …

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge. Read More »