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One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles.

One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles.

In the current examine, we describe a new process using freezing-thawing to density gradient resolution of Nycodenz for one-step separation of organelles from the rat liver and subsequent proteome evaluation of subcellular fractions. To put together two-dimensional electrophoresis (2-D PAGE) profiles of tissue organelles, we carried out one-step subcellular fractionation of rat liver homogenate using …

One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles. Read More »

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

The most potent antigen presenting cell current in peripheral blood, lymphoid and non-lymphoid tissue is the dendritic cell (DC). The research of human DC has been restricted by their low frequency within the tissues and the dearth of a really DC particular floor marker to help in identification and isolation. Standard methods for the isolation …

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation. Read More »