In the current examine, we describe a new process using freezing-thawing to density gradient resolution of Nycodenz for one-step separation of organelles from the rat
Category: Medium & Serums
Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.
In the central nervous system (CNS) peroxisomes are current in all cell varieties, particularly neurons, oligodendrocytes, astrocytes, microglia, and endothelial cells. Brain peroxisomes are smaller
Internalization of intact poliovirus by HeLa cells as shown by subcellular fractionation in isoosmotic Nycodenz gradients.
HeLa cells had been contaminated with radiolabelled poliovirus at completely different temperatures, and the intracellular distribution of enter radioactivity was studied. To this finish, homogenates
Separation of endocytic vesicles in Nycodenz gradients.
The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied utilizing Nycodenz/sucrose gradients. It was proven in pulse chase experiments that the ligand endocytosed initially