States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

In the central nervous system (CNS) peroxisomes are current in all cell varieties, particularly neurons, oligodendrocytes, astrocytes, microglia, and endothelial cells. Brain peroxisomes are smaller in measurement in contrast to peroxisomes from different tissues and are due to this fact referred to as microperoxisomes. We have established a purification process to isolate extremely purified peroxisomes …

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes. Read More »

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

The most potent antigen presenting cell current in peripheral blood, lymphoid and non-lymphoid tissue is the dendritic cell (DC). The research of human DC has been restricted by their low frequency within the tissues and the dearth of a really DC particular floor marker to help in identification and isolation. Standard methods for the isolation …

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation. Read More »

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.

Commonly, subcellular organelles equivalent to nuclei, mitochondria, lysosomes, and Golgi membranes are remoted first by differential centrifugation in low-speed or high-speed centrifuges after which purified by gradient centrifugation in ultracentrifuges. We have ready these organelles using a brand new high-speed centrifuge (28,000 rpm max) which permits the era of larger radial centrifugal forces (rcfs) than …

Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge. Read More »

States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.

States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.

Mature double-stranded DNA bacteriophages have capsids with symmetrical shells that usually resist disruption, as they need to to outlive within the wild. However, flexibility and related dynamism help operate. We describe biochemistry-oriented procedures used to seek out beforehand obscure flexibility for capsids of the associated phages, T3 and T7. The main procedures are hydration-based buoyant …

States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM. Read More »