States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.

States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.

Mature double-stranded DNA bacteriophages have capsids with symmetrical shells that usually resist disruption, as they need to to outlive within the wild. However, flexibility and related dynamism help operate. We describe biochemistry-oriented procedures used to seek out beforehand obscure flexibility for capsids of the associated phages, T3 and T7. The main procedures are hydration-based buoyant …

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