The most potent antigen presenting cell current in peripheral blood, lymphoid and non-lymphoid tissue is the dendritic cell (DC). The research of human DC has been restricted by their low frequency within the tissues and the dearth of a really DC particular floor marker to help in identification and isolation. Standard methods for the isolation of blood DC usually make use of a interval of in vitro tradition adopted by flotation on dense albumin gradients, or extra lately, discontinuous gradients of metrizamide.
Dense albumin gradients are time consuming to organize, giving low and variable yields of DC. Metrizamide is extra handy, though publicity of monocytes to metrizamide can lower the expression of CD14 and alter the accent cell properties of antigen presenting cells. Here we display that Nycodenz gradient centrifugation of 16 h cultured, T lymphocyte depleted, peripheral blood mononuclear cells (PBMC) reliably yields a inhabitants of low density cells that’s extremely enriched for DC.
Most B and residual T lymphocytes are depleted and NK cell numbers are lowered two-fold from the interface cell inhabitants. The excessive density pellet fraction reveals little or no allostimulatory exercise, indicating that few DC cross into the pellet. The low density fraction incorporates a big inhabitants (20 +/- 5 (SD)%, n = 8) of cells which fail to stain for the lineage markers CD3, CD11b, CD14, CD16, CD19 and CD57. Nycodenz reveals low toxicity, doesn’t alter the allostimulatory exercise of antigen presenting cells, and is subsequently supreme for the isolation of cultured DC.
Phytanic acid alpha-oxidation. Differential subcellular localization in rat and human tissues and its inhibition by nycodenz.
The subcellular web site of oxidation of [1-14C]phytanic acid to pristanic acid and CO2 was examined by measurement of the discharge of 14CO2 in several organelles from human and rat tissues ready by isopycnic density gradient centrifugation in Nycodenz. The exercise of phytanic acid oxidation in human tissues (liver and cultured pores and skin fibroblasts) paralleled that of the peroxisomal marker catalase. We additionally noticed that Nycodenz (generally used gradient materials for isolation of subcellular organelles) has a powerful inhibitory impact on the alpha-oxidation of phytanic acid.
This inhibition is reversible and may be decreased or eradicated by dialysis of remoted organelles towards isotonic resolution. The dialysis of endoplasmic reticulum, mitochondrial, and peroxisomal fractions from human liver and cultured pores and skin fibroblasts for two h towards isotonic resolution elevated the precise exercise of phytanic acid oxidation by 1.3-, 1.3-, and 5-21-fold, respectively, after removing of Nycodenz as in contrast with nondialyzed samples.
After dialysis, the speed of oxidation of phytanic acid in peroxisomes from human liver and cultured pores and skin fibroblasts was 4-26 instances greater than that in mitochondria and 43-130 instances than that within the endoplasmic reticulum, suggesting that, in human tissues, phytanic acid is oxidized to pristanic acid in peroxisomes. On the opposite hand, the oxidation of phytanic acid in rat liver paralleled the distribution of the mitochondrial marker cytochrome-c oxidase.
The 18-fold greater fee of oxidation in dialyzed mitochondria (198.6 +/- 4.20 pmol/h/mg of protein) than in peroxisomes (11.0 +/- 0.5 pmol/h/mg of protein) demonstrates that, in rodents, phytanic acid is oxidized in mitochondria. 2-[5-(4-Chlorophenyl)pentyl]oxiran-2-carboxylic acid, an inhibitor of carnitine palmitoyltransferase I and mitochondrial fatty acid oxidation, inhibits the oxidation of phytanic acid in rat tissues (liver and cultured pores and skin fibroblasts), whereas it has no impact on the oxidation of phytanic acid in human tissues (liver and cultured pores and skin fibroblasts).
The greater particular exercise of phytanic acid oxidation in peroxisomes in contrast with that in mitochondria and the endoplasmic reticulum from human tissues and the inhibition of phytanic acid oxidation by 2-[5-(4-chlorophenyl)pentyl]oxiran-2-carboxylic acid in rat tissues (however not human tissues) display clearly that, in human tissues, phytanic acid is predominantly oxidized in peroxisomes.
Reproducible isolation of kind II pneumocytes from fetal and grownup rat lung utilizing nycodenz density gradients.
Isolating recent, comparatively pure kind II pneumocytes from the lung, significantly of fetal origin, is a tough course of. Separation by buoyant density gradient centrifugation has been used efficiently to isolate grownup kind II cells. There is concern, nevertheless, that Percoll, a gradient medium that’s generally used for kind II cell isolation, could also be poisonous to cells. We evaluated a brand new gradient medium, Nycodenz, that’s (1) a real resolution, (2) clear, (3) not metabolized by cells, and (4) unhazardous to cells.
Type II pneumocytes had been remoted from 19- and 21-day gestation fetal and grownup rat lung by elastase digestion and separated on preformed isotonic Nycodenz gradients (2 mL every of 27.6, 20.7, 13.8, and 4.6 (w/v) options). Type II pneumocytes had been recovered from the density vary 1.057-1.061 and recognized by binding of FITC-conjugated and gold-complexed Maclura pomifera lectin. Cells derived from 19-day fetal lung contained ample glycogen and reacted with a monoclonal antibody to the cytokeratins Eight and 18, that are markers of the fetal kind II cell. Adult kind II cells reacted with antibodies to cytokeratins 8, 18, and 19.
Type II cell purity was 79.7 +/- 2.4%, 83.8 +/- 2.8%, and 82.6 +/- 1.8% (means +/- SEM) for 19- and 21-day gestation fetal and grownup lung preparations, respectively. Cell viability was higher than 95%. The remaining cell yield for grownup preparations was 17.8 +/- 2.7 x 10(6)/rat (means +/- SEM). To decide if the freshly remoted kind II pneumocytes had been functionally energetic, the incorporation of [3H]choline into phosphatidylcholine was measured.
The % saturation of phosphatidylcholine was excessive for each populations of freshly remoted cells. However, grownup kind II pneumocytes integrated [3H]choline into phosphatidylcholine extra quickly than 21-day gestation fetal cells (5.97 x 10(-3) dpm/10(6) cells/h vs. 0.32 x 10(-3) dpm/10(6) cells/h, P lower than .005).
 ELISA kit) Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E03I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E03I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E03I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E03I0130-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E03I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E03I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Rat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E02I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E02I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E02I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Rat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E02I0130-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E02I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E02I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Dog ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E08I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E08I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E08I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Dog ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E08I0130-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E08I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E08I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Goat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E06I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E06I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E06I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Goat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E06I0130-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E06I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E06I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E07I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E07I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E07I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E07I0130-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E07I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E07I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E04I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E04I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E04I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E04I0130-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E04I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E04I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E09I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E09I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E09I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E09I0130-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E09I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E09I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E05I0129-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E05I0129-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E05I0129-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 ELISA kit) Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E05I0130-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E05I0130-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E05I0130-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 7079 1 PIPET 1ML NON STERILE NON STERILE |
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| 7079-1N | CORNING | 250/pk | EUR 235 |
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Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line |
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 7079 2 PIPET 2ML NON STERILE NON STERILE |
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| 7079-2N | CORNING | 700/pk | EUR 171 |
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Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line |
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 7079 5 PIPET 5ML NON STERILE NON STERILE |
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| 7079-5N | CORNING | 240/pk | EUR 270 |
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Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line |
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) Goat control serum (non-immunized, non-hemolyzed, delipidized) |
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| NGTS-10D | Alpha Diagnostics | 10 ml | EUR 141 |
) CY2 (Non-Sulfonated) |
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| HY-D0827 | MedChemExpress | 25mg | EUR 969 |
) Goat control serum (non-immunized, non-hemolyzed, heat-inactivated) |
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| NGTS-10H | Alpha Diagnostics | 10 ml | EUR 141 |
 Non-phospho AKT ELISA Kit| Porcine Non-phosphorylated AKT ELISA |
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| EF016713 | Lifescience Market | 96 Tests | EUR 689 |
 Mixed breed) Bovine control serum (non-immunized, non-hemolyzed, delipidized) Mixed breed |
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| NBOS-100D | Alpha Diagnostics | 100 ml | EUR 529 |
) Goat control serum (non-immunized, non-hemolyzed, delipidized, heat-inactivated) |
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| NGTS-10DH | Alpha Diagnostics | 10 ml | EUR 164 |
) Chicken IgY (Non Immune) |
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| 31R-1052 | Fitzgerald | 1 ml | EUR 148 |
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Description: Purified Chicken non immune IgY |
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 Non Filamentous Actin antibody |
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| 10R-2476 | Fitzgerald | 5 mL | EUR 478 |
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Description: Mouse monoclonal Non-Filamentous Actin antibody |
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 Non Neuronal Enolase antibody |
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| 10R-8640 | Fitzgerald | 100 ul | EUR 393 |
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Description: Mouse monoclonal Non Neuronal Enolase antibody |
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Non Neuronal Enolase antibody |
|||
| 10R-8641 | Fitzgerald | 100 ul | EUR 393 |
|
Description: Mouse monoclonal Non Neuronal Enolase antibody |
|||
 Non-secretory ribonuclease Antibody |
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| 20-abx110050 | Abbexa |
|
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 Non-fat Dry Milk |
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| 22012 | Biotium | 100G | EUR 102 |
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Description: Minimum order quantity: 1 unit of 100G |
|||
 Rabbit Serum (Non-Sterile |
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| 20-abx800001 | Abbexa |
|
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Rabbit Serum (Non-Sterile |
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| abx800001-1L | Abbexa | 1 L | EUR 4281 |
) Sheep Serum (Non-Sterile) |
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| 20-abx800018 | Abbexa |
|
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Sheep Serum (Non-Sterile) |
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| abx800018-1L | Abbexa | 1 L | EUR 746 |
) Goat Serum (Non-Sterile) |
|||
| 20-abx800033 | Abbexa |
|
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Goat Serum (Non-Sterile) |
|||
| abx800033-1L | Abbexa | 1 L | EUR 759 |
) Horse Serum (Non-Sterile) |
|||
| 20-abx800048 | Abbexa |
|
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Horse Serum (Non-Sterile) |
|||
| abx800048-1L | Abbexa | 1 L | EUR 1080 |
) Donkey Serum (Non-Sterile) |
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| 20-abx800063 | Abbexa |
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Donkey Serum (Non-Sterile) |
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| abx800063-1L | Abbexa | 1 L | EUR 1803 |
) Camel Serum (Non-Sterile) |
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| 20-abx800078 | Abbexa |
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Camel Serum (Non-Sterile) |
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| abx800078-1L | Abbexa | 1 L | EUR 4699 |
) Dog Serum (Non-Sterile) |
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| 20-abx800108 | Abbexa |
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Dog Serum (Non-Sterile) |
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| abx800108-1L | Abbexa | 1 L | EUR 3919 |
) Chicken Serum (Non-Sterile) |
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| 20-abx800123 | Abbexa |
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Chicken Serum (Non-Sterile) |
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| abx800123-1L | Abbexa | 1 L | EUR 2054 |
 Non-Neuronal Enolase Antibody |
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| abx020643-1mg | Abbexa | 1 mg | EUR 815 |
 Cholesterol, non-animal origin |
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| GE7110-1G | Glentham Life Sciences | 1 g | EUR 229 |
 Non-Hodgkin lymphoma Exosome |
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| P141-NH | 101Bio | - | Ask for price |
 Non-Fat Powdered Milk |
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| NB0669 | Bio Basic | 250g | EUR 65.66 |
) Cy 3 (Non-Sulfonated) |
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| HY-D0968 | MedChemExpress | 1mg | EUR 497 |
) Cy3 azide (non-sulfonated) |
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| A8111-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient. |
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Cy3 azide (non-sulfonated) |
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| A8111-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient. |
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) Cy3.5 azide (non-sulfonated) |
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| A8112-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines |
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Cy3.5 azide (non-sulfonated) |
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| A8112-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines |
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) Cy5 azide (non-sulfonated) |
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| A8113-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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Cy5 azide (non-sulfonated) |
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| A8113-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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) Cy5.5 azide (non-sulfonated) |
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| A8114-25 | ApexBio | 25 mg | EUR 1616 |
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Description: This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680. |
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Cy5.5 azide (non-sulfonated) |
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| A8114-5 | ApexBio | 5 mg | EUR 583 |
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Description: This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680. |
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) Cy7 azide (non-sulfonated) |
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| A8115-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility. |
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Cy7 azide (non-sulfonated) |
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| A8115-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility. |
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) Cy7.5 azide (non-sulfonated) |
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| A8116-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used. |
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Cy7.5 azide (non-sulfonated) |
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| A8116-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used. |
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) Cy3 alkyne (non-sulfonated) |
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| A8130-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it. |
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Cy3 alkyne (non-sulfonated) |
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| A8130-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it. |
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) Cy5 alkyne (non-sulfonated) |
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| A8131-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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Cy5 alkyne (non-sulfonated) |
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| A8131-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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) Cy3 maleimide (non-sulfonated) |
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| A8138-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). |
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Cy3 maleimide (non-sulfonated) |
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| A8138-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). |
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) Cy5 maleimide (non-sulfonated) |
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| A8139-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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Cy5 maleimide (non-sulfonated) |
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| A8139-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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) Cy5.5 maleimide (non-sulfonated) |
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| A8140-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications. |
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Cy5.5 maleimide (non-sulfonated) |
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| A8140-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications. |
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) Cy7 maleimide (non-sulfonated) |
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| A8141-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. |
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Cy7 maleimide (non-sulfonated) |
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| A8141-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. |
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) Cy7.5 maleimide(non-sulfonated) |
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| A8142-25 | ApexBio | 25 mg | EUR 525 |
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Description: Thiol-reactive, near infrared dye Cy7.5 for the labeling of protein SH groups.Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near infrared (NIR) fluorescent conjugates. |
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Cy7.5 maleimide(non-sulfonated) |
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| A8142-5 | ApexBio | 5 mg | EUR 293 |
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Description: Thiol-reactive, near infrared dye Cy7.5 for the labeling of protein SH groups.Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near infrared (NIR) fluorescent conjugates. |
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) Cy5 amine (non-sulfonated) |
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| A8143-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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Cy5 amine (non-sulfonated) |
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| A8143-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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) Cy5 hydrazide (non-sulfonated) |
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| A8145-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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Cy5 hydrazide (non-sulfonated) |
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| A8145-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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) Cy5.5 hydrazide (non-sulfonated) |
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| A8261-1 | ApexBio | 1 mg | EUR 177 |
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Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. |
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Cy5.5 hydrazide (non-sulfonated) |
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| A8261-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. |
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We have demonstrated that, utilizing the Nycodenz isolation technique, it’s attainable to acquire a excessive yield of comparatively pure viable kind II cells from fetal and grownup lung that can be utilized for speedy research or cultured with a superb plating effectivity (39 +/- 6.3%).