The most potent antigen presenting cell current in peripheral blood, lymphoid and non-lymphoid tissue is the dendritic cell (DC). The research of human DC has been restricted by their low frequency within the tissues and the dearth of a really DC particular floor marker to help in identification and isolation. Standard methods for the isolation of blood DC usually make use of a interval of in vitro tradition adopted by flotation on dense albumin gradients, or extra lately, discontinuous gradients of metrizamide.
Dense albumin gradients are time consuming to organize, giving low and variable yields of DC. Metrizamide is extra handy, though publicity of monocytes to metrizamide can lower the expression of CD14 and alter the accent cell properties of antigen presenting cells. Here we display that Nycodenz gradient centrifugation of 16 h cultured, T lymphocyte depleted, peripheral blood mononuclear cells (PBMC) reliably yields a inhabitants of low density cells that’s extremely enriched for DC.
Most B and residual T lymphocytes are depleted and NK cell numbers are lowered two-fold from the interface cell inhabitants. The excessive density pellet fraction reveals little or no allostimulatory exercise, indicating that few DC cross into the pellet. The low density fraction incorporates a big inhabitants (20 +/- 5 (SD)%, n = 8) of cells which fail to stain for the lineage markers CD3, CD11b, CD14, CD16, CD19 and CD57. Nycodenz reveals low toxicity, doesn’t alter the allostimulatory exercise of antigen presenting cells, and is subsequently supreme for the isolation of cultured DC.
Phytanic acid alpha-oxidation. Differential subcellular localization in rat and human tissues and its inhibition by nycodenz.
The subcellular web site of oxidation of [1-14C]phytanic acid to pristanic acid and CO2 was examined by measurement of the discharge of 14CO2 in several organelles from human and rat tissues ready by isopycnic density gradient centrifugation in Nycodenz. The exercise of phytanic acid oxidation in human tissues (liver and cultured pores and skin fibroblasts) paralleled that of the peroxisomal marker catalase. We additionally noticed that Nycodenz (generally used gradient materials for isolation of subcellular organelles) has a powerful inhibitory impact on the alpha-oxidation of phytanic acid.
This inhibition is reversible and may be decreased or eradicated by dialysis of remoted organelles towards isotonic resolution. The dialysis of endoplasmic reticulum, mitochondrial, and peroxisomal fractions from human liver and cultured pores and skin fibroblasts for two h towards isotonic resolution elevated the precise exercise of phytanic acid oxidation by 1.3-, 1.3-, and 5-21-fold, respectively, after removing of Nycodenz as in contrast with nondialyzed samples.
After dialysis, the speed of oxidation of phytanic acid in peroxisomes from human liver and cultured pores and skin fibroblasts was 4-26 instances greater than that in mitochondria and 43-130 instances than that within the endoplasmic reticulum, suggesting that, in human tissues, phytanic acid is oxidized to pristanic acid in peroxisomes. On the opposite hand, the oxidation of phytanic acid in rat liver paralleled the distribution of the mitochondrial marker cytochrome-c oxidase.
The 18-fold greater fee of oxidation in dialyzed mitochondria (198.6 +/- 4.20 pmol/h/mg of protein) than in peroxisomes (11.0 +/- 0.5 pmol/h/mg of protein) demonstrates that, in rodents, phytanic acid is oxidized in mitochondria. 2-[5-(4-Chlorophenyl)pentyl]oxiran-2-carboxylic acid, an inhibitor of carnitine palmitoyltransferase I and mitochondrial fatty acid oxidation, inhibits the oxidation of phytanic acid in rat tissues (liver and cultured pores and skin fibroblasts), whereas it has no impact on the oxidation of phytanic acid in human tissues (liver and cultured pores and skin fibroblasts).
The greater particular exercise of phytanic acid oxidation in peroxisomes in contrast with that in mitochondria and the endoplasmic reticulum from human tissues and the inhibition of phytanic acid oxidation by 2-[5-(4-chlorophenyl)pentyl]oxiran-2-carboxylic acid in rat tissues (however not human tissues) display clearly that, in human tissues, phytanic acid is predominantly oxidized in peroxisomes.
Reproducible isolation of kind II pneumocytes from fetal and grownup rat lung utilizing nycodenz density gradients.
Isolating recent, comparatively pure kind II pneumocytes from the lung, significantly of fetal origin, is a tough course of. Separation by buoyant density gradient centrifugation has been used efficiently to isolate grownup kind II cells. There is concern, nevertheless, that Percoll, a gradient medium that’s generally used for kind II cell isolation, could also be poisonous to cells. We evaluated a brand new gradient medium, Nycodenz, that’s (1) a real resolution, (2) clear, (3) not metabolized by cells, and (4) unhazardous to cells.
Type II pneumocytes had been remoted from 19- and 21-day gestation fetal and grownup rat lung by elastase digestion and separated on preformed isotonic Nycodenz gradients (2 mL every of 27.6, 20.7, 13.8, and 4.6 (w/v) options). Type II pneumocytes had been recovered from the density vary 1.057-1.061 and recognized by binding of FITC-conjugated and gold-complexed Maclura pomifera lectin. Cells derived from 19-day fetal lung contained ample glycogen and reacted with a monoclonal antibody to the cytokeratins Eight and 18, that are markers of the fetal kind II cell. Adult kind II cells reacted with antibodies to cytokeratins 8, 18, and 19.
Type II cell purity was 79.7 +/- 2.4%, 83.8 +/- 2.8%, and 82.6 +/- 1.8% (means +/- SEM) for 19- and 21-day gestation fetal and grownup lung preparations, respectively. Cell viability was higher than 95%. The remaining cell yield for grownup preparations was 17.8 +/- 2.7 x 10(6)/rat (means +/- SEM). To decide if the freshly remoted kind II pneumocytes had been functionally energetic, the incorporation of [3H]choline into phosphatidylcholine was measured.
The % saturation of phosphatidylcholine was excessive for each populations of freshly remoted cells. However, grownup kind II pneumocytes integrated [3H]choline into phosphatidylcholine extra quickly than 21-day gestation fetal cells (5.97 x 10(-3) dpm/10(6) cells/h vs. 0.32 x 10(-3) dpm/10(6) cells/h, P lower than .005).
Ionic Strength Adjustor Fluoride TISAB3 - 500ML |
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| TISAF5 | Scientific Laboratory Supplies | 500ML | EUR 140.4 |
Ionic Strength Adjustor Lead 2.5M NaNO3 - 500ML |
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| ISAPB5 | Scientific Laboratory Supplies | 500ML | EUR 246.76 |
Ionic Strength Adjustor Barium 4M LiCl - 500ML |
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| ISABA5 | Scientific Laboratory Supplies | 500ML | EUR 186.54 |
Ionic Strength Adjustor Calcium 4M KCI - 500ML |
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| ISACA5 | Scientific Laboratory Supplies | 500ML | EUR 142.47 |
Ionic Strength Adjustor Silver 5M NaNO3 - 500ML |
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| ISAAG5 | Scientific Laboratory Supplies | 500ML | EUR 227.66 |
Ionic Strength Adjustor Copper 5M NaNO3 - 500ML |
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| ISACU5 | Scientific Laboratory Supplies | 500ML | EUR 142.47 |
Ionic Strength Adjustor Iodide 5M NaNO3 - 500ML |
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| ISAI5 | Scientific Laboratory Supplies | 500ML | EUR 259.2 |
Ionic Strength Adjustor Bromide 5M NaNO3 - 500ML |
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| ISABR5 | Scientific Laboratory Supplies | 500ML | EUR 201.23 |
Ionic Strength Adjustor Cadmium 5M NaNO3 - 500ML |
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| ISACD5 | Scientific Laboratory Supplies | 500ML | EUR 186.54 |
Ionic Strength Adjustor Cyanide 10M NaOH - 500ML |
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| ISACN5 | Scientific Laboratory Supplies | 500ML | EUR 186.54 |
Ionic Strength Adjustor Mercury 5M NaNO3 - 500ML |
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| ISAHG5 | Scientific Laboratory Supplies | 500ML | EUR 136.6 |
Ionic Strength Adjustor Magnesium 4M KCl - 500ML |
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| ISAMG5 | Scientific Laboratory Supplies | 500ML | EUR 146.88 |
Ionic Strength Adjustor Sodium Based Std - 500ML |
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| ISANA5 | Scientific Laboratory Supplies | 500ML | EUR 165.97 |
Ionic Strength Adjustor Ammonium 4M LiCl - 500ML |
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| ISANH45 | Scientific Laboratory Supplies | 500ML | EUR 450.92 |
Ionic Strength Adjustor Ammonia 10M NaOH - 500ML |
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| ISANH5 | Scientific Laboratory Supplies | 500ML | EUR 444.15 |
Ionic Strength Adjustor Chloride 5M NaNO3 - 500ML |
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| ISACL5 | Scientific Laboratory Supplies | 500ML | EUR 140.4 |
Ionic Strength Adjustor Potassium 5M NaCl - 500ML |
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| ISAK5 | Scientific Laboratory Supplies | 500ML | EUR 262.92 |
Ionic Strength Adjustor Sulphide 10M NaOH - 500ML |
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| ISAS5 | Scientific Laboratory Supplies | 500ML | EUR 186.54 |
Ionic Strength Adjustor Thiocyanate 5M NaNO3 - 500ML |
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| ISASC5 | Scientific Laboratory Supplies | 500ML | EUR 186.54 |
Ionic Strength Adjustor Carbon Dioxide Solution - 500ML |
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| ISACO5 | Scientific Laboratory Supplies | 500ML | EUR 146.88 |
pH Buff Sol 4.10 +/- 0.04 at 20C (Low Ionic St) - 5L |
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| LS415 | Scientific Laboratory Supplies | 5L | EUR 193.05 |
Ionic Strength Adjustor Nitrate 2M (NH4)2SO4 - 500ML |
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| ISAN5 | Scientific Laboratory Supplies | 500ML | EUR 259.2 |
Ionic Strength Adjustor Water Hardness 4M KCl - 500ML |
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| ISAWH5 | Scientific Laboratory Supplies | 500ML | EUR 144.45 |
Ionic Strength Adjustor Perchlorate 2M (NH4)2SO4 - 500ML |
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| ISAPCL5 | Scientific Laboratory Supplies | 500ML | EUR 186.54 |
Ionic Strength Adjustor Sulphur Dioxide 2M H2SO4 - 500ML |
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| ISASO5 | Scientific Laboratory Supplies | 500ML | EUR 186.54 |
Elga Labpure S3 Purification Cartridge - Low Ionic - EACH |
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| WAT4030 | Scientific Laboratory Supplies | EACH | EUR 712.37 |
Rat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E02I0129-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E02I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E02I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E02I0130-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E02I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E02I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E08I0129-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E08I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E08I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E08I0130-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E08I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E08I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E07I0129-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E07I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E07I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E07I0130-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E07I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E07I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E01A14063 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Rat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E01A14064 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
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Goat ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E01A48972 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
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Goat ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E01A48973 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
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Goat ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E06I0129-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E06I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E06I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E06I0130-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E06I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E06I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Electrode Filling Sol Low Ionic Strength Applications - 100ML |
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| EFSLIAPP | Scientific Laboratory Supplies | 100ML | EUR 114.57 |
Sheep ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E01A101265 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Sheep ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E01A101266 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
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Human ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01A5311 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
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Human ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01A5312 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
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Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E03I0129-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E03I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E03I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E03I0130-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E03I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E03I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01I0129-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Human ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Human ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Human ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01I0130-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Human ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Human ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Human ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Human ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01A22805 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01A22806 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Canine ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01A66403 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Canine ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01A66404 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01A31540 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01A31541 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01A75119 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01A75120 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Bovine ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E01A83837 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Bovine ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E01A83838 | BlueGene | 96T | EUR 700 |
|
Description: ELISA |
|||
Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E09I0129-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E09I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit |
|||
| E09I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit |
|||
| E09I0130-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E09I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E09I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E04I0129-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E04I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E04I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E04I0130-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E04I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E04I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Porcine ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E01A57690 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Porcine ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E01A57691 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Chicken ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E01A92557 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Chicken ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E01A92558 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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SurfactAway™ Non-ionic |
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| SA890-250 | Biotech Support Group | 250 mL | EUR 1666.8 |
SurfactAway™ Non-ionic |
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| SA890-30 | Biotech Support Group | 30 mL | EUR 495 |
Guinea Pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E01A40253 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Guinea Pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E01A40254 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E05I0129-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E05I0129-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit |
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| E05I0129-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E05I0130-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E05I0130-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit |
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| E05I0130-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Low Ionic St Elect 2 Buffers (pH 4.10 and pH 6.96) Kit - 1KIT |
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| LSKIT1 | Scientific Laboratory Supplies | 1KIT | EUR 154.22 |
Non-Ionic Polymer Absorbent_x000D__x000D_ |
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| A16729 | Pfaltz & Bauer | 25G | EUR 231.55 |
Non-Ionic Surfactants Cuvette Test - EACH |
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| LCK333 | Scientific Laboratory Supplies | EACH | EUR 256.5 |
5M Sodium Nitrate Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes - 500ML |
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| IS-BUF1-500 | Scientific Laboratory Supplies | 500ML | EUR 178.2 |
10M Sodium Hydroxide Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes - 500ML |
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| IS-BUF2-500 | Scientific Laboratory Supplies | 500ML | EUR 207.9 |
Low Level TISAB II Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes - 500ML |
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| IS-BUF3-500 | Scientific Laboratory Supplies | 500ML | EUR 175.5 |
Amberlite@ XAD-4 Non-Ionic Polymeric Adsorbent 20-40 mesh |
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| A16731 | Pfaltz & Bauer | 500G | EUR 145.5 |
(untagged)-Human cDNA FLJ31250 fis, clone KIDNE2005336, weakly similar to Human antigen NY-CO-31 (NY-CO-31) mRNA |
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| SC312057 | Origene Technologies GmbH | 10 µg | Ask for price |
NY-CO-1 Polyclonal Antibody |
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| E20-73215 | EnoGene | 100ug | EUR 225 |
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Description: Available in various conjugation types. |
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We have demonstrated that, utilizing the Nycodenz isolation technique, it’s attainable to acquire a excessive yield of comparatively pure viable kind II cells from fetal and grownup lung that can be utilized for speedy research or cultured with a superb plating effectivity (39 +/- 6.3%).
