Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

The most potent antigen presenting cell current in peripheral blood, lymphoid and non-lymphoid tissue is the dendritic cell (DC). The research of human DC has been restricted by their low frequency within the tissues and the dearth of a really DC particular floor marker to help in identification and isolation. Standard methods for the isolation of blood DC usually make use of a interval of in vitro tradition adopted by flotation on dense albumin gradients, or extra lately, discontinuous gradients of metrizamide.
Dense albumin gradients are time consuming to organize, giving low and variable yields of DC. Metrizamide is extra handy, though publicity of monocytes to metrizamide can lower the expression of CD14 and alter the accent cell properties of antigen presenting cells. Here we display that Nycodenz gradient centrifugation of 16 h cultured, T lymphocyte depleted, peripheral blood mononuclear cells (PBMC) reliably yields a inhabitants of low density cells that’s extremely enriched for DC.
Most B and residual T lymphocytes are depleted and NK cell numbers are lowered two-fold from the interface cell inhabitants. The excessive density pellet fraction reveals little or no allostimulatory exercise, indicating that few DC cross into the pellet. The low density fraction incorporates a big inhabitants (20 +/- 5 (SD)%, n = 8) of cells which fail to stain for the lineage markers CD3, CD11b, CD14, CD16, CD19 and CD57. Nycodenz reveals low toxicity, doesn’t alter the allostimulatory exercise of antigen presenting cells, and is subsequently supreme for the isolation of cultured DC.

Phytanic acid alpha-oxidation. Differential subcellular localization in rat and human tissues and its inhibition by nycodenz.

The subcellular web site of oxidation of [1-14C]phytanic acid to pristanic acid and CO2 was examined by measurement of the discharge of 14CO2 in several organelles from human and rat tissues ready by isopycnic density gradient centrifugation in Nycodenz. The exercise of phytanic acid oxidation in human tissues (liver and cultured pores and skin fibroblasts) paralleled that of the peroxisomal marker catalase. We additionally noticed that Nycodenz (generally used gradient materials for isolation of subcellular organelles) has a powerful inhibitory impact on the alpha-oxidation of phytanic acid.
This inhibition is reversible and may be decreased or eradicated by dialysis of remoted organelles towards isotonic resolution. The dialysis of endoplasmic reticulum, mitochondrial, and peroxisomal fractions from human liver and cultured pores and skin fibroblasts for two h towards isotonic resolution elevated the precise exercise of phytanic acid oxidation by 1.3-, 1.3-, and 5-21-fold, respectively, after removing of Nycodenz as in contrast with nondialyzed samples.
After dialysis, the speed of oxidation of phytanic acid in peroxisomes from human liver and cultured pores and skin fibroblasts was 4-26 instances greater than that in mitochondria and 43-130 instances than that within the endoplasmic reticulum, suggesting that, in human tissues, phytanic acid is oxidized to pristanic acid in peroxisomes. On the opposite hand, the oxidation of phytanic acid in rat liver paralleled the distribution of the mitochondrial marker cytochrome-c oxidase.
The 18-fold greater fee of oxidation in dialyzed mitochondria (198.6 +/- 4.20 pmol/h/mg of protein) than in peroxisomes (11.0 +/- 0.5 pmol/h/mg of protein) demonstrates that, in rodents, phytanic acid is oxidized in mitochondria. 2-[5-(4-Chlorophenyl)pentyl]oxiran-2-carboxylic acid, an inhibitor of carnitine palmitoyltransferase I and mitochondrial fatty acid oxidation, inhibits the oxidation of phytanic acid in rat tissues (liver and cultured pores and skin fibroblasts), whereas it has no impact on the oxidation of phytanic acid in human tissues (liver and cultured pores and skin fibroblasts).
The greater particular exercise of phytanic acid oxidation in peroxisomes in contrast with that in mitochondria and the endoplasmic reticulum from human tissues and the inhibition of phytanic acid oxidation by 2-[5-(4-chlorophenyl)pentyl]oxiran-2-carboxylic acid in rat tissues (however not human tissues) display clearly that, in human tissues, phytanic acid is predominantly oxidized in peroxisomes.

Reproducible isolation of kind II pneumocytes from fetal and grownup rat lung utilizing nycodenz density gradients.

Isolating recent, comparatively pure kind II pneumocytes from the lung, significantly of fetal origin, is a tough course of. Separation by buoyant density gradient centrifugation has been used efficiently to isolate grownup kind II cells. There is concern, nevertheless, that Percoll, a gradient medium that’s generally used for kind II cell isolation, could also be poisonous to cells. We evaluated a brand new gradient medium, Nycodenz, that’s (1) a real resolution, (2) clear, (3) not metabolized by cells, and (4) unhazardous to cells.
Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.
Type II pneumocytes had been remoted from 19- and 21-day gestation fetal and grownup rat lung by elastase digestion and separated on preformed isotonic Nycodenz gradients (2 mL every of 27.6, 20.7, 13.8, and 4.6 (w/v) options). Type II pneumocytes had been recovered from the density vary 1.057-1.061 and recognized by binding of FITC-conjugated and gold-complexed Maclura pomifera lectin. Cells derived from 19-day fetal lung contained ample glycogen and reacted with a monoclonal antibody to the cytokeratins Eight and 18, that are markers of the fetal kind II cell. Adult kind II cells reacted with antibodies to cytokeratins 8, 18, and 19.
Type II cell purity was 79.7 +/- 2.4%, 83.8 +/- 2.8%, and 82.6 +/- 1.8% (means +/- SEM) for 19- and 21-day gestation fetal and grownup lung preparations, respectively. Cell viability was higher than 95%. The remaining cell yield for grownup preparations was 17.8 +/- 2.7 x 10(6)/rat (means +/- SEM). To decide if the freshly remoted kind II pneumocytes had been functionally energetic, the incorporation of [3H]choline into phosphatidylcholine was measured.
The % saturation of phosphatidylcholine was excessive for each populations of freshly remoted cells. However, grownup kind II pneumocytes integrated [3H]choline into phosphatidylcholine extra quickly than 21-day gestation fetal cells (5.97 x 10(-3) dpm/10(6) cells/h vs. 0.32 x 10(-3) dpm/10(6) cells/h, P lower than .005).

Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit

E03I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit

E03I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse ionic amino acid transporter 3(SLC7A3) ELISA kit

E03I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit

E03I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit

E03I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse ionic amino acid transporter 4(SLC7A4) ELISA kit

E03I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat ionic amino acid transporter 3(SLC7A3) ELISA kit

E02I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat ionic amino acid transporter 3(SLC7A3) ELISA kit

E02I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat ionic amino acid transporter 3(SLC7A3) ELISA kit

E02I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat ionic amino acid transporter 4(SLC7A4) ELISA kit

E02I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat ionic amino acid transporter 4(SLC7A4) ELISA kit

E02I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat ionic amino acid transporter 4(SLC7A4) ELISA kit

E02I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog ionic amino acid transporter 3(SLC7A3) ELISA kit

E08I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog ionic amino acid transporter 3(SLC7A3) ELISA kit

E08I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog ionic amino acid transporter 3(SLC7A3) ELISA kit

E08I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog ionic amino acid transporter 4(SLC7A4) ELISA kit

E08I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog ionic amino acid transporter 4(SLC7A4) ELISA kit

E08I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog ionic amino acid transporter 4(SLC7A4) ELISA kit

E08I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat ionic amino acid transporter 3(SLC7A3) ELISA kit

E06I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat ionic amino acid transporter 3(SLC7A3) ELISA kit

E06I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat ionic amino acid transporter 3(SLC7A3) ELISA kit

E06I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat ionic amino acid transporter 4(SLC7A4) ELISA kit

E06I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat ionic amino acid transporter 4(SLC7A4) ELISA kit

E06I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat ionic amino acid transporter 4(SLC7A4) ELISA kit

E06I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig ionic amino acid transporter 3(SLC7A3) ELISA kit

E07I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig ionic amino acid transporter 3(SLC7A3) ELISA kit

E07I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig ionic amino acid transporter 3(SLC7A3) ELISA kit

E07I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig ionic amino acid transporter 4(SLC7A4) ELISA kit

E07I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig ionic amino acid transporter 4(SLC7A4) ELISA kit

E07I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig ionic amino acid transporter 4(SLC7A4) ELISA kit

E07I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit

E04I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit

E04I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit ionic amino acid transporter 3(SLC7A3) ELISA kit

E04I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit

E04I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit

E04I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit ionic amino acid transporter 4(SLC7A4) ELISA kit

E04I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit

E09I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit

E09I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey ionic amino acid transporter 3(SLC7A3) ELISA kit

E09I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit

E09I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit

E09I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey ionic amino acid transporter 4(SLC7A4) ELISA kit

E09I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit

E05I0129-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit

E05I0129-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig ionic amino acid transporter 3(SLC7A3) ELISA kit

E05I0129-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 3(SLC7A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit

E05I0130-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit

E05I0130-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig ionic amino acid transporter 4(SLC7A4) ELISA kit

E05I0130-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Guinea pig ionic amino acid transporter 4(SLC7A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

7079 1 PIPET 1ML NON STERILE NON STERILE

7079-1N 250/pk
EUR 235
Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line

7079 2 PIPET 2ML NON STERILE NON STERILE

7079-2N 700/pk
EUR 171
Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line

7079 5 PIPET 5ML NON STERILE NON STERILE

7079-5N 240/pk
EUR 270
Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line

Goat control serum (non-immunized, non-hemolyzed, delipidized)

NGTS-10D 10 ml
EUR 141

CY2 (Non-Sulfonated)

HY-D0827 25mg
EUR 969

Goat control serum (non-immunized, non-hemolyzed, heat-inactivated)

NGTS-10H 10 ml
EUR 141

Non-phospho AKT ELISA Kit| Porcine Non-phosphorylated AKT ELISA

EF016713 96 Tests
EUR 689

Bovine control serum (non-immunized, non-hemolyzed, delipidized) Mixed breed

NBOS-100D 100 ml
EUR 529

Goat control serum (non-immunized, non-hemolyzed, delipidized, heat-inactivated)

NGTS-10DH 10 ml
EUR 164

Chicken IgY (Non Immune)

31R-1052 1 ml
EUR 148
Description: Purified Chicken non immune IgY

Non Filamentous Actin antibody

10R-2476 5 mL
EUR 478
Description: Mouse monoclonal Non-Filamentous Actin antibody

Non Neuronal Enolase antibody

10R-8640 100 ul
EUR 393
Description: Mouse monoclonal Non Neuronal Enolase antibody

Non Neuronal Enolase antibody

10R-8641 100 ul
EUR 393
Description: Mouse monoclonal Non Neuronal Enolase antibody

Non-secretory ribonuclease Antibody

20-abx110050
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Non-fat Dry Milk

22012 100G
EUR 102
Description: Minimum order quantity: 1 unit of 100G

Rabbit Serum (Non-Sterile

20-abx800001
  • EUR 1080.00
  • EUR 2472.00
  • EUR 662.00
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Non-Sterile

abx800001-1L 1 L
EUR 4281

Sheep Serum (Non-Sterile)

20-abx800018
  • EUR 481.00
  • EUR 592.00
  • EUR 370.00
  • 100 ml
  • 500 ml
  • 50 ml

Sheep Serum (Non-Sterile)

abx800018-1L 1 L
EUR 746

Goat Serum (Non-Sterile)

20-abx800033
  • EUR 495.00
  • EUR 606.00
  • EUR 370.00
  • 100 ml
  • 500 ml
  • 50 ml

Goat Serum (Non-Sterile)

abx800033-1L 1 L
EUR 759

Horse Serum (Non-Sterile)

20-abx800048
  • EUR 648.00
  • EUR 829.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml

Horse Serum (Non-Sterile)

abx800048-1L 1 L
EUR 1080

Donkey Serum (Non-Sterile)

20-abx800063
  • EUR 1038.00
  • EUR 1372.00
  • EUR 648.00
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Serum (Non-Sterile)

abx800063-1L 1 L
EUR 1803

Camel Serum (Non-Sterile)

20-abx800078
  • EUR 1149.00
  • EUR 2722.00
  • EUR 787.00
  • 100 ml
  • 500 ml
  • 50 ml

Camel Serum (Non-Sterile)

abx800078-1L 1 L
EUR 4699

Dog Serum (Non-Sterile)

20-abx800108
  • EUR 996.00
  • EUR 2263.00
  • EUR 620.00
  • 100 ml
  • 500 ml
  • 50 ml

Dog Serum (Non-Sterile)

abx800108-1L 1 L
EUR 3919

Chicken Serum (Non-Sterile)

20-abx800123
  • EUR 578.00
  • EUR 1233.00
  • EUR 398.00
  • 100 ml
  • 500 ml
  • 50 ml

Chicken Serum (Non-Sterile)

abx800123-1L 1 L
EUR 2054

Non-Neuronal Enolase Antibody

abx020643-1mg 1 mg
EUR 815

Cholesterol, non-animal origin

GE7110-1G 1 g
EUR 229

Non-Hodgkin lymphoma Exosome

P141-NH - Ask for price

Non-Fat Powdered Milk

NB0669 250g
EUR 65.66

Cy 3 (Non-Sulfonated)

HY-D0968 1mg
EUR 497

Cy3 azide (non-sulfonated)

A8111-25 25 mg
EUR 1616
Description: Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient.

Cy3 azide (non-sulfonated)

A8111-5 5 mg
EUR 583
Description: Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient.

Cy3.5 azide (non-sulfonated)

A8112-25 25 mg
EUR 1616
Description: Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines

Cy3.5 azide (non-sulfonated)

A8112-5 5 mg
EUR 583
Description: Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines

Cy5 azide (non-sulfonated)

A8113-25 25 mg
EUR 1616
Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction.

Cy5 azide (non-sulfonated)

A8113-5 5 mg
EUR 583
Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction.

Cy5.5 azide (non-sulfonated)

A8114-25 25 mg
EUR 1616
Description: This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680.

Cy5.5 azide (non-sulfonated)

A8114-5 5 mg
EUR 583
Description: This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680.

Cy7 azide (non-sulfonated)

A8115-25 25 mg
EUR 1616
Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility.

Cy7 azide (non-sulfonated)

A8115-5 5 mg
EUR 583
Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility.

Cy7.5 azide (non-sulfonated)

A8116-25 25 mg
EUR 1616
Description: Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used.

Cy7.5 azide (non-sulfonated)

A8116-5 5 mg
EUR 583
Description: Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used.

Cy3 alkyne (non-sulfonated)

A8130-25 25 mg
EUR 1616
Description: Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it.

Cy3 alkyne (non-sulfonated)

A8130-5 5 mg
EUR 583
Description: Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it.

Cy5 alkyne (non-sulfonated)

A8131-25 25 mg
EUR 1616
Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore.

Cy5 alkyne (non-sulfonated)

A8131-5 5 mg
EUR 583
Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore.

Cy3 maleimide (non-sulfonated)

A8138-25 25 mg
EUR 525
Description: Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides).

Cy3 maleimide (non-sulfonated)

A8138-5 5 mg
EUR 293
Description: Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides).

Cy5 maleimide (non-sulfonated)

A8139-25 25 mg
EUR 525
Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups.

Cy5 maleimide (non-sulfonated)

A8139-5 5 mg
EUR 293
Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups.

Cy5.5 maleimide (non-sulfonated)

A8140-25 25 mg
EUR 525
Description: Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications.

Cy5.5 maleimide (non-sulfonated)

A8140-5 5 mg
EUR 293
Description: Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications.

Cy7 maleimide (non-sulfonated)

A8141-25 25 mg
EUR 525
Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups.

Cy7 maleimide (non-sulfonated)

A8141-5 5 mg
EUR 293
Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups.

Cy7.5 maleimide(non-sulfonated)

A8142-25 25 mg
EUR 525
Description: Thiol-reactive, near infrared dye Cy7.5 for the labeling of protein SH groups.Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near infrared (NIR) fluorescent conjugates.

Cy7.5 maleimide(non-sulfonated)

A8142-5 5 mg
EUR 293
Description: Thiol-reactive, near infrared dye Cy7.5 for the labeling of protein SH groups.Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near infrared (NIR) fluorescent conjugates.

Cy5 amine (non-sulfonated)

A8143-25 25 mg
EUR 525
Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents.

Cy5 amine (non-sulfonated)

A8143-5 5 mg
EUR 293
Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents.

Cy5 hydrazide (non-sulfonated)

A8145-25 25 mg
EUR 525
Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones.

Cy5 hydrazide (non-sulfonated)

A8145-5 5 mg
EUR 293
Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones.

Cy5.5 hydrazide (non-sulfonated)

A8261-1 1 mg
EUR 177
Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage.

Cy5.5 hydrazide (non-sulfonated)

A8261-25 25 mg
EUR 525
Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage.
We have demonstrated that, utilizing the Nycodenz isolation technique, it’s attainable to acquire a excessive yield of comparatively pure viable kind II cells from fetal and grownup lung that can be utilized for speedy research or cultured with a superb plating effectivity (39 +/- 6.3%).