Porcine reproductive and respiratory syndrome (PRRS) is a significant concern in the swine industry, leading to reproductive issues and economic losses. AffiVET® Rapid Test kits
Author: Leonard Gilbert
One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles.
In the current examine, we describe a new process using freezing-thawing to density gradient resolution of Nycodenz for one-step separation of organelles from the rat
Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.
In the central nervous system (CNS) peroxisomes are current in all cell varieties, particularly neurons, oligodendrocytes, astrocytes, microglia, and endothelial cells. Brain peroxisomes are smaller
Internalization of intact poliovirus by HeLa cells as shown by subcellular fractionation in isoosmotic Nycodenz gradients.
HeLa cells had been contaminated with radiolabelled poliovirus at completely different temperatures, and the intracellular distribution of enter radioactivity was studied. To this finish, homogenates
Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.
The most potent antigen presenting cell current in peripheral blood, lymphoid and non-lymphoid tissue is the dendritic cell (DC). The research of human DC has
Separation of endocytic vesicles in Nycodenz gradients.
The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied utilizing Nycodenz/sucrose gradients. It was proven in pulse chase experiments that the ligand endocytosed initially
Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.
Commonly, subcellular organelles equivalent to nuclei, mitochondria, lysosomes, and Golgi membranes are remoted first by differential centrifugation in low-speed or high-speed centrifuges after which purified
States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.
Mature double-stranded DNA bacteriophages have capsids with symmetrical shells that usually resist disruption, as they need to to outlive within the wild. However, flexibility and
A novel technique to prepare a single cell suspension of isolated quiescent human hepatic stellate cells.
To discover a easy and easy-to-learn process for the isolation of human quiescent hepatic stellate cells (HSCs) that requires no superior coaching. Thus decreasing prices
Studies of dihydroxyacetone phosphate acyltransferase in rat small intestine. Subcellular localization and effect of partially hydrogenated fish oil and clofibrate.
The subcellular localization of dihydroxyacetone phosphate acyltransferase (DHAPAT) exercise in rat small gut was investigated by Nycodenz-gradient centrifugation. We discovered that DHAPAT had a predominant peroxisomal