In the central nervous system (CNS) peroxisomes are current in all cell varieties, particularly neurons, oligodendrocytes, astrocytes, microglia, and endothelial cells. Brain peroxisomes are smaller in measurement in contrast to peroxisomes from different tissues and are due to this fact referred to as microperoxisomes. We have established a purification process to isolate extremely purified peroxisomes from the central nervous system which might be nicely separated from the endoplasmic reticulum and mitochondria and are free of myelin contamination.
The main issue in purification of mind peroxisomes in contrast to peroxisomes from liver or kidney is the presence of massive quantities of myelin in the CNS, which leads to contamination of the subcellular fractions. Hence, the essential step of the isolation process is the elimination of myelin by the use of a sucrose gradient, since with out the elimination of myelin no vital enrichment of purified peroxisomes may be achieved. Another issue is that in mind tissue the abundance of peroxisomes decreases considerably throughout postnatal improvement. We present a detailed protocol for the isolation of peroxisomes from mouse central nervous system in addition to a protocol for the isolation of peroxisomes from the liver and kidney utilizing a steady Nycodenz gradient.
Isolation of Plasmodium berghei ookinetes in tradition utilizing Nycodenz density gradient columns and magnetic isolation.
BACKGROUND: Large scale in vitro manufacturing of the mosquito levels of malaria parasites stays elusive, with solely restricted success for full sporogonic improvement and just one report of improvement by to infective sporozoites. The preliminary step on this course of is the manufacturing, in vitro, of ookinetes from gametocytaemic blood. Methods for isolation of these ookinetes from blood cells have been described; nevertheless, as well as to yield typically being low, processing time and potential for contamination by erythrocytes stay excessive.
METHODS: This research compares two procedures for retaining mature ookinetes from blood stage cultures, while eradicating crimson blood cells and different contaminants prior to additional tradition of the parasite. The nicely established methodology of isolation on Nycodenz cushions is in contrast with a novel methodology using the innate magnetic properties of the haem pigment crystals present in the cytoplasm of ookinetes. RESULTS: Yield and viability of ookinetes had been comparable with each isolation strategies.
However, in our arms magnetic isolation produced a cleaner ookinete preparation far more rapidly. Moreover, lowering the stream charge by the magnetic column may additional improve the yield. CONCLUSION: We suggest the enrichment of an ookinete preparation prior to additional tradition being carried out utilizing the magnetic properties of Plasmodium berghei ookinetes in its place to their density. The former approach is quicker, removes extra erythrocytes, however day-to-day prices are larger.
Efficient purification of neonatal porcine gonocytes with Nycodenz and differential plating.
Gonocytes are the solely sort of germ cells current in the postnatal testis and give rise to spermatogonial stem cells. Purification of gonocytes has necessary implications for the research and manipulation of these cells and could present insights for the ongoing investigation of the male germline stem cells. To receive a pure inhabitants of gonocytes from piglet testis cells, a wide selection of Nycodenz concentrations had been investigated for density gradient centrifugation.
We additionally examined differential plating of testis cells for varied tradition durations with completely different extracellular matrix (ECM) parts (fibronectin, poly-d-lysine, poly-L-lysine, laminin and collagen Types I and IV). Gonocytes had been extremely enriched in pellets of testis cells after utilizing 17% Nycodenz centrifugation to a purity of 81±9%.
After culturing testis cells on plates precoated with completely different ECM parts for 120 min, the proportion of gonocytes elevated amongst non-adherent cells (suspended in the medium), with fibronectin or poly-D-lysine leading to the best (up to 85%) and laminin in the lowest (54%) gonocyte proportion. Combining the most promising ECM coatings (fibronectin and poly-d-lysine) and additional extension of their tradition length to 240 min didn’t enhance ultimate gonocyte purity.
However, centrifugation with 17% Nycodenz adopted by differential plating with fibronectin and poly-d-lysine coating additional purified gonocytes amongst the collected cells to >90%. These outcomes present a easy, fast and environment friendly strategy for acquiring extremely enriched populations of piglet gonocytes to be used in the research and manipulation of these germline stem cells.
Separation and dedication of nycodenz and its impurities by capillary electrophoresis
Separation and dedication of nycodenz (a new radiography reagent) and its impurities by capillary electrophoresis was investigated. Since the analytes had been polyols, Na2B4O7-H3BO3 was chosen as the buffer system. The analytes had been negatively charged by complexing with borate. Then cetyltrimethylammonium bromide was used to reverse electroosmotic mobility so as to shorten the evaluation time. 2-Propanol was added to optimize the separation additional. Acceptable outcomes of linearity, precision of corrected peak space and migration time had been obtained. The RSD(n = 6) of corrected peak space and migration time had been 0.73% and 1.4% respectively. The outcomes obtained by CE had been immediately in contrast to these obtained by excessive efficiency liquid chromatography.
Purification of mouse primordial germ cells by Nycodenz.
Primordial germ cells are necessary cells for the research of germ cell lineage. It has proved troublesome to receive extremely purified primordial germ cells for preparation of a particular antibody. In the current research, a new methodology for purifying mouse primordial germ cells was developed utilizing a Nycodenz gradient. Furthermore, the polyclonal anti-mouse primordial germ cells IgG derived from mouse primordial germ cells was ready. As this IgG reacted solely with primordial germ cells obtained at day 12.5 after mating, this antibody appeared to acknowledge the stage-specific antigen of primordial germ cells.
One motive that a steady primordial germ cell marker has not been obtained is as a result of the purity of the primordial germ cells used has been too low to put together the antibody. This new methodology represents a vital enchancment in the purification of primordial germ cells; it’s less complicated than earlier strategies, and produced mouse primordial germ cells with a purity of greater than 95%.
 Mouse Toxic shock syndrome toxin 1 ELISA kit |
|||
| E03T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Rat Toxic shock syndrome toxin 1 ELISA kit |
|||
| E02T0564-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rat Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rat Toxic shock syndrome toxin 1 ELISA kit |
|||
| E02T0564-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rat Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rat Toxic shock syndrome toxin 1 ELISA kit |
|||
| E02T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rat Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
) Staphylococcus aureus Toxic shock syndrome toxin-1 (tst) |
|||
| 1-CSB-EP361973FKZ | Cusabio |
|
|
|
Description: Recombinant Staphylococcus aureus Toxic shock syndrome toxin-1(tst) expressed in E.coli |
|||
 Goat Toxic shock syndrome toxin 1 ELISA kit |
|||
| E06T0564-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Goat Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat Toxic shock syndrome toxin 1 ELISA kit |
|||
| E06T0564-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Goat Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Goat Toxic shock syndrome toxin 1 ELISA kit |
|||
| E06T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Goat Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Dog Toxic shock syndrome toxin 1 ELISA kit |
|||
| E08T0564-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Canine Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Dog Toxic shock syndrome toxin 1 ELISA kit |
|||
| E08T0564-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Canine Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Dog Toxic shock syndrome toxin 1 ELISA kit |
|||
| E08T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Canine Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Pig Toxic shock syndrome toxin 1 ELISA kit |
|||
| E07T0564-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Porcine Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig Toxic shock syndrome toxin 1 ELISA kit |
|||
| E07T0564-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Porcine Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Pig Toxic shock syndrome toxin 1 ELISA kit |
|||
| E07T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Porcine Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Rabbit Toxic shock syndrome toxin 1 ELISA kit |
|||
| E04T0564-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rabbit Toxic shock syndrome toxin 1 ELISA kit |
|||
| E04T0564-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Rabbit Toxic shock syndrome toxin 1 ELISA kit |
|||
| E04T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Monkey Toxic shock syndrome toxin 1 ELISA kit |
|||
| E09T0564-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey Toxic shock syndrome toxin 1 ELISA kit |
|||
| E09T0564-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Monkey Toxic shock syndrome toxin 1 ELISA kit |
|||
| E09T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Monkey Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 ELISA Kit) Toxic Shock Syndrome Toxin (TSST-1) ELISA Kit |
|||
| abx053645-96tests | Abbexa | 96 tests | EUR 668 |
 Mouse toxic shock syndrome toxin,TSST-1 ELISA Kit |
|||
| CN-02872M1 | ChemNorm | 96T | EUR 471 |
Mouse toxic shock syndrome toxin,TSST-1 ELISA Kit |
|||
| CN-02872M2 | ChemNorm | 48T | EUR 322 |
 Guinea pig Toxic shock syndrome toxin 1 ELISA kit |
|||
| E05T0564-192T | BlueGene | 192 tests | EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Guinea pig Toxic shock syndrome toxin 1 ELISA kit |
|||
| E05T0564-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
Guinea pig Toxic shock syndrome toxin 1 ELISA kit |
|||
| E05T0564-96 | BlueGene | 1 plate of 96 wells | EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Guinea pig Toxic shock syndrome toxin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
|||
 Human toxic shock syndrome toxin,TSST-1 ELISA Kit |
|||
| 201-12-0351 | SunredBio | 96 tests | EUR 440 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
|||
ELISA Kit) Mouse toxic shock syndrome toxin(TSST-1)ELISA Kit |
|||
| GA-E0313MS-48T | GenAsia Biotech | 48T | EUR 336 |
Mouse toxic shock syndrome toxin(TSST-1)ELISA Kit |
|||
| GA-E0313MS-96T | GenAsia Biotech | 96T | EUR 534 |
ELISA Kit) Human toxic shock syndrome toxin(TSST-1)ELISA Kit |
|||
| GA-E0367HM-48T | GenAsia Biotech | 48T | EUR 289 |
Human toxic shock syndrome toxin(TSST-1)ELISA Kit |
|||
| GA-E0367HM-96T | GenAsia Biotech | 96T | EUR 466 |
 (58-78)) Toxic Shock Syndrome Toxin-1 (TSST-1) (58-78) |
|||
| H-2522.0500 | Bachem | 0.5mg | EUR 683 |
|
Description: Sum Formula: C98H171N33O35; CAS# [137593-46-5] |
|||
Toxic Shock Syndrome Toxin-1 (TSST-1) (58-78) |
|||
| H-2522.1000 | Bachem | 1.0mg | EUR 1204 |
|
Description: Sum Formula: C98H171N33O35; CAS# [137593-46-5] |
|||
Human toxic shock syndrome toxin(TSST-1)ELISA Kit |
|||
| QY-E03774 | Qayee Biotechnology | 96T | EUR 361 |
 7079 1 PIPET 1ML NON STERILE NON STERILE |
|||
| 7079-1N | CORNING | 250/pk | EUR 235 |
|
Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line |
|||
 7079 2 PIPET 2ML NON STERILE NON STERILE |
|||
| 7079-2N | CORNING | 700/pk | EUR 171 |
|
Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line |
|||
 7079 5 PIPET 5ML NON STERILE NON STERILE |
|||
| 7079-5N | CORNING | 240/pk | EUR 270 |
|
Description: Disposable Pipets; Disposable Pipets, CGW, Serological, Std Line |
|||
) Goat control serum (non-immunized, non-hemolyzed, delipidized) |
|||
| NGTS-10D | Alpha Diagnostics | 10 ml | EUR 141 |
) CY2 (Non-Sulfonated) |
|||
| HY-D0827 | MedChemExpress | 25mg | EUR 969 |
) Goat control serum (non-immunized, non-hemolyzed, heat-inactivated) |
|||
| NGTS-10H | Alpha Diagnostics | 10 ml | EUR 141 |
 Recombinant S. aureus Toxic shock syndrome toxin-1 Protein, His-SUMO, E.coli-100ug |
|||
| QP7369-ec-100ug | EnQuireBio | 100ug | EUR 707 |
 Recombinant S. aureus Toxic shock syndrome toxin-1 Protein, His-SUMO, E.coli-10ug |
|||
| QP7369-ec-10ug | EnQuireBio | 10ug | EUR 326 |
 Recombinant S. aureus Toxic shock syndrome toxin-1 Protein, His-SUMO, E.coli-1mg |
|||
| QP7369-ec-1mg | EnQuireBio | 1mg | EUR 2303 |
 Recombinant S. aureus Toxic shock syndrome toxin-1 Protein, His-SUMO, E.coli-200ug |
|||
| QP7369-ec-200ug | EnQuireBio | 200ug | EUR 1115 |
 Recombinant S. aureus Toxic shock syndrome toxin-1 Protein, His-SUMO, E.coli-500ug |
|||
| QP7369-ec-500ug | EnQuireBio | 500ug | EUR 1514 |
 Recombinant S. aureus Toxic shock syndrome toxin-1 Protein, His-SUMO, E.coli-50ug |
|||
| QP7369-ec-50ug | EnQuireBio | 50ug | EUR 435 |
 Non-phospho AKT ELISA Kit| Porcine Non-phosphorylated AKT ELISA |
|||
| EF016713 | Lifescience Market | 96 Tests | EUR 689 |
 Mixed breed) Bovine control serum (non-immunized, non-hemolyzed, delipidized) Mixed breed |
|||
| NBOS-100D | Alpha Diagnostics | 100 ml | EUR 529 |
) Goat control serum (non-immunized, non-hemolyzed, delipidized, heat-inactivated) |
|||
| NGTS-10DH | Alpha Diagnostics | 10 ml | EUR 164 |
) Chicken IgY (Non Immune) |
|||
| 31R-1052 | Fitzgerald | 1 ml | EUR 148 |
|
Description: Purified Chicken non immune IgY |
|||
 Non Filamentous Actin antibody |
|||
| 10R-2476 | Fitzgerald | 5 mL | EUR 478 |
|
Description: Mouse monoclonal Non-Filamentous Actin antibody |
|||
 Non Neuronal Enolase antibody |
|||
| 10R-8640 | Fitzgerald | 100 ul | EUR 393 |
|
Description: Mouse monoclonal Non Neuronal Enolase antibody |
|||
Non Neuronal Enolase antibody |
|||
| 10R-8641 | Fitzgerald | 100 ul | EUR 393 |
|
Description: Mouse monoclonal Non Neuronal Enolase antibody |
|||
 Non-secretory ribonuclease Antibody |
|||
| 20-abx110050 | Abbexa |
|
|
 Non-fat Dry Milk |
|||
| 22012 | Biotium | 100G | EUR 102 |
|
Description: Minimum order quantity: 1 unit of 100G |
|||
 Rabbit Serum (Non-Sterile |
|||
| 20-abx800001 | Abbexa |
|
|
Rabbit Serum (Non-Sterile |
|||
| abx800001-1L | Abbexa | 1 L | EUR 4281 |
) Sheep Serum (Non-Sterile) |
|||
| 20-abx800018 | Abbexa |
|
|
Sheep Serum (Non-Sterile) |
|||
| abx800018-1L | Abbexa | 1 L | EUR 746 |
) Goat Serum (Non-Sterile) |
|||
| 20-abx800033 | Abbexa |
|
|
Goat Serum (Non-Sterile) |
|||
| abx800033-1L | Abbexa | 1 L | EUR 759 |
) Horse Serum (Non-Sterile) |
|||
| 20-abx800048 | Abbexa |
|
|
Horse Serum (Non-Sterile) |
|||
| abx800048-1L | Abbexa | 1 L | EUR 1080 |
) Donkey Serum (Non-Sterile) |
|||
| 20-abx800063 | Abbexa |
|
|
Donkey Serum (Non-Sterile) |
|||
| abx800063-1L | Abbexa | 1 L | EUR 1803 |
) Camel Serum (Non-Sterile) |
|||
| 20-abx800078 | Abbexa |
|
|
Camel Serum (Non-Sterile) |
|||
| abx800078-1L | Abbexa | 1 L | EUR 4699 |
) Dog Serum (Non-Sterile) |
|||
| 20-abx800108 | Abbexa |
|
|
Dog Serum (Non-Sterile) |
|||
| abx800108-1L | Abbexa | 1 L | EUR 3919 |
) Chicken Serum (Non-Sterile) |
|||
| 20-abx800123 | Abbexa |
|
|
Chicken Serum (Non-Sterile) |
|||
| abx800123-1L | Abbexa | 1 L | EUR 2054 |
 Non-Neuronal Enolase Antibody |
|||
| abx020643-1mg | Abbexa | 1 mg | EUR 815 |
 Cholesterol, non-animal origin |
|||
| GE7110-1G | Glentham Life Sciences | 1 g | EUR 229 |
 Non-Hodgkin lymphoma Exosome |
|||
| P141-NH | 101Bio | - | Ask for price |
 Non-Fat Powdered Milk |
|||
| NB0669 | Bio Basic | 250g | EUR 65.66 |
) Cy 3 (Non-Sulfonated) |
|||
| HY-D0968 | MedChemExpress | 1mg | EUR 497 |
) Cy3 azide (non-sulfonated) |
|||
| A8111-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient. |
|||
Cy3 azide (non-sulfonated) |
|||
| A8111-5 | ApexBio | 5 mg | EUR 583 |
|
Description: Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient. |
|||
) Cy3.5 azide (non-sulfonated) |
|||
| A8112-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines |
|||
Cy3.5 azide (non-sulfonated) |
|||
| A8112-5 | ApexBio | 5 mg | EUR 583 |
|
Description: Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines |
|||
) Cy5 azide (non-sulfonated) |
|||
| A8113-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
|||
Cy5 azide (non-sulfonated) |
|||
| A8113-5 | ApexBio | 5 mg | EUR 583 |
|
Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
|||
) Cy5.5 azide (non-sulfonated) |
|||
| A8114-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680. |
|||
Cy5.5 azide (non-sulfonated) |
|||
| A8114-5 | ApexBio | 5 mg | EUR 583 |
|
Description: This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680. |
|||
) Cy7 azide (non-sulfonated) |
|||
| A8115-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility. |
|||
Cy7 azide (non-sulfonated) |
|||
| A8115-5 | ApexBio | 5 mg | EUR 583 |
|
Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility. |
|||
) Cy7.5 azide (non-sulfonated) |
|||
| A8116-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used. |
|||
Cy7.5 azide (non-sulfonated) |
|||
| A8116-5 | ApexBio | 5 mg | EUR 583 |
|
Description: Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used. |
|||
) Cy3 alkyne (non-sulfonated) |
|||
| A8130-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it. |
|||
Cy3 alkyne (non-sulfonated) |
|||
| A8130-5 | ApexBio | 5 mg | EUR 583 |
|
Description: Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it. |
|||
) Cy5 alkyne (non-sulfonated) |
|||
| A8131-25 | ApexBio | 25 mg | EUR 1616 |
|
Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
|||
Cy5 alkyne (non-sulfonated) |
|||
| A8131-5 | ApexBio | 5 mg | EUR 583 |
|
Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
|||
) Cy3 maleimide (non-sulfonated) |
|||
| A8138-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). |
|||
Cy3 maleimide (non-sulfonated) |
|||
| A8138-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). |
|||
) Cy5 maleimide (non-sulfonated) |
|||
| A8139-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
|||
Cy5 maleimide (non-sulfonated) |
|||
| A8139-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
|||
) Cy5.5 maleimide (non-sulfonated) |
|||
| A8140-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications. |
|||
Cy5.5 maleimide (non-sulfonated) |
|||
| A8140-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications. |
|||
) Cy7 maleimide (non-sulfonated) |
|||
| A8141-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. |
|||
Cy7 maleimide (non-sulfonated) |
|||
| A8141-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. |
|||
) Cy7.5 maleimide(non-sulfonated) |
|||
| A8142-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Thiol-reactive, near infrared dye Cy7.5 for the labeling of protein SH groups.Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near infrared (NIR) fluorescent conjugates. |
|||
Cy7.5 maleimide(non-sulfonated) |
|||
| A8142-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Thiol-reactive, near infrared dye Cy7.5 for the labeling of protein SH groups.Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near infrared (NIR) fluorescent conjugates. |
|||
) Cy5 amine (non-sulfonated) |
|||
| A8143-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
|||
Cy5 amine (non-sulfonated) |
|||
| A8143-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
|||
) Cy5 hydrazide (non-sulfonated) |
|||
| A8145-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
|||
Cy5 hydrazide (non-sulfonated) |
|||
| A8145-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
|||
) Cy5.5 hydrazide (non-sulfonated) |
|||
| A8261-1 | ApexBio | 1 mg | EUR 177 |
|
Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. |
|||
Cy5.5 hydrazide (non-sulfonated) |
|||
| A8261-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. |
|||
Cy5.5 hydrazide (non-sulfonated) |
|||
| A8261-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. |
|||
Cy5.5 hydrazide (non-sulfonated) |
|||
| A8261-50 | ApexBio | 50 mg | EUR 856 |
|
Description: Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. |
|||
) Cy3.5 maleimide (non-sulfonated) |
|||
| A8264-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy3.5 maleimide can be used for selective labeling of free sulfhydryl groups (e.g. cysteine residues in peptides, proteins and oligonucleotides). Before labeling, cystines should be prepared with reductant such as TCEP (tris-carboxyethylphosphine). |
|||
Cy3.5 maleimide (non-sulfonated) |
|||
| A8264-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy3.5 maleimide can be used for selective labeling of free sulfhydryl groups (e.g. cysteine residues in peptides, proteins and oligonucleotides). Before labeling, cystines should be prepared with reductant such as TCEP (tris-carboxyethylphosphine). |
|||
) Cy3 hydrazide (non-sulfonated) |
|||
| A8265-1 | ApexBio | 1 mg | EUR 177 |
|
Description: Cy3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide. Cyanine3 dyes have been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. |
|||
Cy3 hydrazide (non-sulfonated) |
|||
| A8265-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide. Cyanine3 dyes have been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. |
|||
Cy3 hydrazide (non-sulfonated) |
|||
| A8265-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide. Cyanine3 dyes have been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. |
|||
Cy3 hydrazide (non-sulfonated) |
|||
| A8265-50 | ApexBio | 50 mg | EUR 856 |
|
Description: Cy3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide. Cyanine3 dyes have been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. |
|||
) Cy3.5 hydrazide (non-sulfonated) |
|||
| A8266-1 | ApexBio | 1 mg | EUR 177 |
|
Description: Cy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. |
|||
Cy3.5 hydrazide (non-sulfonated) |
|||
| A8266-25 | ApexBio | 25 mg | EUR 525 |
|
Description: Cy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. |
|||
Cy3.5 hydrazide (non-sulfonated) |
|||
| A8266-5 | ApexBio | 5 mg | EUR 293 |
|
Description: Cy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. |
|||
Cy3.5 hydrazide (non-sulfonated) |
|||
| A8266-50 | ApexBio | 50 mg | EUR 856 |
|
Description: Cy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. |
|||
 Non-sterile canine serum |
|||
| CAS05-0100 | Equitech | 100 ml | EUR 335.4 |
Non-sterile canine serum |
|||
| CAS05-0500 | Equitech | 500 ml | EUR 508.3 |
 Non-sterile chicken serum |
|||
| CS05-0100 | Equitech | 100 ml | EUR 158.6 |
Non-sterile chicken serum |
|||
| CS05-0500 | Equitech | 500 ml | EUR 188.5 |
 Non-sterile calf serum |
|||
| BCSU05-0100 | Equitech | 100 ml | EUR 145.6 |
Non-sterile calf serum |
|||
| BCSU05-0500 | Equitech | 500 ml | EUR 163.8 |
 Non-sterile bovine serum |
|||
| BSU05-0100 | Equitech | 100 ml | EUR 145.6 |
Non-sterile bovine serum |
|||
| BSU05-0500 | Equitech | 500 ml | EUR 163.8 |
 Non-sterile donkey serum |
|||
| DS05-0100 | Equitech | 100 ml | EUR 161.2 |
Non-sterile donkey serum |
|||
| DS05-0500 | Equitech | 500 ml | EUR 182 |
 CCK Octapeptide, non-sulfated |
|||
| B5058-1 | ApexBio | 1 mg | EUR 116 |
 Non-sterile mouse serum |
|||
| MS05-0050 | Equitech | 50 ml | EUR 231.4 |
Non-sterile mouse serum |
|||
| MS05-0100 | Equitech | 100 ml | EUR 257.4 |
Non-sterile mouse serum |
|||
| MS05-0500 | Equitech | 500 ml | EUR 440.7 |
 Non-sterile feline serum |
|||
| FS05-0100 | Equitech | 100 ml | EUR 427.7 |
Non-sterile feline serum |
|||
| FS05-0500 | Equitech | 500 ml | EUR 1134.9 |
 Non-sterile goat serum |
|||
| GS05-0100 | Equitech | 100 ml | EUR 161.2 |
Non-sterile goat serum |
|||
| GS05-0500 | Equitech | 500 ml | EUR 182 |
 Non-sterile hamster serum |
|||
| HAS05-0050 | Equitech | 50 ml | EUR 257.4 |
Non-sterile hamster serum |
|||
| HAS05-0100 | Equitech | 100 ml | EUR 327.6 |
Non-sterile hamster serum |
|||
| HAS05-0500 | Equitech | 500 ml | EUR 603.2 |
 Non-sterile equine serum |
|||
| ES05-0100 | Equitech | 100 ml | EUR 161.2 |
Non-sterile equine serum |
|||
| ES05-0500 | Equitech | 500 ml | EUR 182 |
 Non-sterile sheep serum |
|||
| OS05-0100 | Equitech | 100 ml | EUR 175.5 |
Non-sterile sheep serum |
|||
| OS05-0500 | Equitech | 500 ml | EUR 223.6 |
 Non-sterile porcine serum |
|||
| PS05-0100 | Equitech | 100ml | EUR 166.4 |
Non-sterile porcine serum |
|||
| PS05-0500 | Equitech | 500 ml | EUR 192.4 |
 Non-sterile rabbit serum |
|||
| RS05-0100 | Equitech | 100 ml | EUR 166.4 |
Non-sterile rabbit serum |
|||
| RS05-0500 | Equitech | 500ml | EUR 192.4 |
 Non-sterile rat serum |
|||
| RTS05-0050 | Equitech | 50 ml | EUR 231.4 |
Non-sterile rat serum |
|||
| RTS05-0100 | Equitech | 100 ml | EUR 257.4 |
Non-sterile rat serum |
|||
| RTS05-0500 | Equitech | 500 ml | EUR 440.7 |
 SurfactAway™ Non-ionic |
|||
| SA890-250 | Biotech Support Group | 250 mL | EUR 1389 |
SurfactAway™ Non-ionic |
|||
| SA890-30 | Biotech Support Group | 30 mL | EUR 379 |
 Mixed breed (non-hemolyzed, heat-inactivated)) Pig serum (control, non-immunized) Mixed breed (non-hemolyzed, heat-inactivated) |
|||
| NPIS-100H | Alpha Diagnostics | 100 ml | EUR 263 |
 New Zealand White (delipidized, non-hemolyzed)) Rabbit control serum (non-immunized) New Zealand White (delipidized, non-hemolyzed) |
|||
| NRBS-100D | Alpha Diagnostics | 100 ml | EUR 347 |
Rabbit control serum (non-immunized) New Zealand White (delipidized, non-hemolyzed) |
|||
| NRBS-10D | Alpha Diagnostics | 10 ml | EUR 202 |
 50µL HAMILTON TIP, CLEAR, NON-FILTERED, NON-STERILE, NESTED TIP RACK STACK |
|||
| HT-50-C-STK | CORNING | 384/pk | EUR 728 |
|
Description: Robotic Tips; Robotic tips - Axygen |
|||
 Mixed breed) Bovine control serum (non-immunized, non-hemolyzed, delipidized, heat-inactivated) Mixed breed |
|||
| NBOS-100DH | Alpha Diagnostics | 100 ml | EUR 529 |
 Mixed breed (non-hemolyzed, heat-inactivated, delipidized)) Pig serum (control, non-immunized) Mixed breed (non-hemolyzed, heat-inactivated, delipidized) |
|||
| NPIS-100HD | Alpha Diagnostics | 100 ml | EUR 286 |
 New Zealand White (heat-inactivated, non-hemolyzed)) Rabbit control serum (non-immunized) New Zealand White (heat-inactivated, non-hemolyzed) |
|||
| NRBS-100H | Alpha Diagnostics | 10 ml | EUR 347 |
Rabbit control serum (non-immunized) New Zealand White (heat-inactivated, non-hemolyzed) |
|||
| NRBS-10H | Alpha Diagnostics | 10 ml | EUR 202 |
 Non Sterile LH Human Plasma |
|||
| 90-1017 | Fitzgerald | 250 ml | EUR 851 |
|
Description: Non Sterile Lithium Heparin Human Plasma |
|||
) Recombinant Enolase, Non Neuronal (NNE) |
|||
| 4-RPB449Bo01 | Cloud-Clone |
|
|
|
Description: Recombinant Bovine Enolase, Non Neuronal expressed in: E.coli |
|||
Recombinant Enolase, Non Neuronal (NNE) |
|||
| 4-RPB449Hu01 | Cloud-Clone |
|
|
|
Description: Recombinant Human Enolase, Non Neuronal expressed in: E.coli |
|||
Recombinant Enolase, Non Neuronal (NNE) |
|||
| 4-RPB449Hu02 | Cloud-Clone |
|
|
|
Description: Recombinant Human Enolase, Non Neuronal expressed in: E.coli |
|||
In addition, the separation reagent Nycodenz is non-toxic and achieved separation of primordial germ cells with out attachment of antibodies in opposition to the primordial germ cell membrane floor. This new purification methodology and stage-specific antibody will likely be helpful for the evaluation of the mechanisms of primordial germ cell migration.