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One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles.

One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles.

In the current examine, we describe a new process using freezing-thawing to density gradient resolution of Nycodenz for one-step separation of organelles from the rat liver and subsequent proteome evaluation of subcellular fractions. To put together two-dimensional electrophoresis (2-D PAGE) profiles of tissue organelles, we carried out one-step subcellular fractionation of rat liver homogenate using …

One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles. Read More »

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

In the central nervous system (CNS) peroxisomes are current in all cell varieties, particularly neurons, oligodendrocytes, astrocytes, microglia, and endothelial cells. Brain peroxisomes are smaller in measurement in contrast to peroxisomes from different tissues and are due to this fact referred to as microperoxisomes. We have established a purification process to isolate extremely purified peroxisomes …

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes. Read More »

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.

The most potent antigen presenting cell current in peripheral blood, lymphoid and non-lymphoid tissue is the dendritic cell (DC). The research of human DC has been restricted by their low frequency within the tissues and the dearth of a really DC particular floor marker to help in identification and isolation. Standard methods for the isolation …

Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation. Read More »

Separation of endocytic vesicles in Nycodenz gradients.

Separation of endocytic vesicles in Nycodenz gradients.

The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied utilizing Nycodenz/sucrose gradients. It was proven in pulse chase experiments that the ligand endocytosed initially (after half of to 1 min) was in small, slow-sedimenting vesicles of comparable sizes. The vesicles containing the ligand elevated in measurement, and after about 2.5 min 20-30% of the …

Separation of endocytic vesicles in Nycodenz gradients. Read More »