In the current examine, we describe a new process using freezing-thawing to density gradient resolution of Nycodenz for one-step separation of organelles from the rat
Category: Exosomes
Internalization of intact poliovirus by HeLa cells as shown by subcellular fractionation in isoosmotic Nycodenz gradients.
HeLa cells had been contaminated with radiolabelled poliovirus at completely different temperatures, and the intracellular distribution of enter radioactivity was studied. To this finish, homogenates
Separation of endocytic vesicles in Nycodenz gradients.
The endocytosis of 125I-labeled asialofetuin by rat hepatocytes was studied utilizing Nycodenz/sucrose gradients. It was proven in pulse chase experiments that the ligand endocytosed initially
Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge.
Commonly, subcellular organelles equivalent to nuclei, mitochondria, lysosomes, and Golgi membranes are remoted first by differential centrifugation in low-speed or high-speed centrifuges after which purified